Page 681 - GreenChemistry2023 Emhetgel

5.11. Chemical technological study on extracting biological active substances (bin - 5) preventing
diabetes, Purevjav Urjintseren, Mongolian State University of Education Mathematics School
of Natural Sciences, Department of Chemistry

Abstract
Recently, there has been common trend among people to refuse from food and medications

produced via synthetic method, but try to consume natural products as much as possible instead.

In this regard, wild berries and medicinal plants are considered to be highly essential for human
health as these kinds of plants serve as rich sources of biological active substances - phenol

compounds. As a result of conducting research on source and spread of herbs which are

commonly used as anti-diabetic medication, we have developed a technological method to
extract preparations from medicinal herbs such as Peony (Paeonia lactiflora Pall), Dandelion

(Taraxacum officinalis Wigg.), Huckleberry (Vaccinium myrtillus L), Blueberry (Vaccinium

uliginosum L), Cranberry (Vaccinium vitisidaea L), and Stinging nettles (Urtica dioica),

accordingly studied chemical composition and antioxidant activity, and conducted
pharmacological study. With the use of Folin Denis & Folin Ciocalteu reagent method,it was

determined that the content of polyphenol compounds was 4.14-5.17% and 27.5 – 101.5mg/ml.

The study was also aimed to investigate DPPH * free radical-scavenging activity in connection
with term, temperature and concentration to identify the most rational technological procedure.

As a result of study, it was identified that free radical-scavenging activity of herbs selected for

the study was generally estimated at 564.25-1750.00 mcg/ml, whereas antioxidant activity of

solvents with 2-10 mg/ml concentration was 417.20-1750.00 mcg /ml, respectively. This shows
that such activity is dependent on concentration. However, in temperature of 30 – 100 0 С

degrees, their activity has slowly been decreased by 1750 mcg/ml – 476.7mcg/ml depending on

temperature. Regarding the stinging nettles, the activity was grown directly dependent from
temperature. DPHH* free radical-scavenging activity was gradually increased in 1-10 minutes,

but was relatively stable and active in 11-16 minutes.