Page 508 - GreenChemistry2023 Emhetgel

Figure 1. Stems and needles seed anatomy Figure 2. Phenolic compounds,
of Juniperus Sabina L. flavonoids and coumarin chromatogram
Materials and methods: The research was carried out in the phytochemical laboratory of the Institute of
Traditional Medicine and Technology. The Juniperus Sabina L was collected from the area of the province
between August and September 2021, dried, and prepared according to standards. The taxonomy and
anatomical structure of the plant were determined by members, a taxonomist at the Botanical Garden of
Scientists of the Institute of Botany of the Mongolian Academy of Sciences. Phytochemical studies were
performed using thin-layer chromatography (TLC) and ultraviolet (UV) spectrophotometry.
Table 1. Description of bioactive ingredients of Juniperus sabina L. in UV spectra

№ Biologically active Standard Equation of Straight Line Results, (%)
compounds substances

1 Polyphenolic Gallic acid y=0.0017 х +0.001 14.07±0.017%
2
composition R =0.9994
2 Total flavonoids Rutin y=41.719 х +0.0063 1.86±0.0076%
R =0.9999
2
3 Total coumarin Umbelliferon y=185.99 х +0.0212 0.38±0.001%
R =0.9990
2
Conclusions: Juniperus Sabina L contains mainly phenolic compounds, flavonoids, and coumarins.

REFERENCES
[1] Shengnan Xu, Xinru Li, Shi Liu, Peilin Tian and Dengwu Li.
Juniperus sabina L. as a Source of Podophyllotoxins: ExtractionOptimization and Anticholinesterase Activities. 2022
Sep 6;23(18):10205. doi: 10.3390/ijms231810205.
[2] Dongye Lu.; Haiguang Huang.; Aijun Wang.; Guosheng Zhang. Genetic Evaluation of Juniperus sabina L.
(Cupressaceae) in Arid and Semi-Arid Regions of China Based on SSR Markers Forests 2022, 13(2),
231; https://doi.org/10.3390/f13020231
[3] Doga Kavaz & Razan El Faraj. Investigation of composition, antioxidant, antimicrobial and cytotoxic
characteristics from Juniperus sabina and Ferula communis extracts May 2023 Scientific Reports 13(1)
DOI:10.1038/s41598-023-34281-x License CC BY 4.0